S100A9 anticorps (AA 67-79)
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- Antigène Voir toutes S100A9 Anticorps
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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Épitope
- AA 67-79
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp S100A9 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human S100A9.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%) Panda (85%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic peptide C-DTNADKQLSFEEF from an internal region of human S100A9 (NP_002956.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%), Panda (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product S100A9 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280(50):41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- avoid freeze thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Antigène
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- Autre désignation
- S100A9 / MRP14 (S100A9 Produits)
- Synonymes
- anticorps 60B8AG, anticorps CAGB, anticorps CFAG, anticorps CGLB, anticorps L1AG, anticorps LIAG, anticorps MAC387, anticorps MIF, anticorps MRP14, anticorps NIF, anticorps P14, anticorps Mrp14, anticorps S100A9, anticorps MRP-14, anticorps p14, anticorps 60B8Ag, anticorps AW546964, anticorps BEE22, anticorps Cagb, anticorps GAGB, anticorps L1Ag, anticorps S100 calcium binding protein A9, anticorps S100 calcium binding protein A9 (calgranulin B), anticorps S100A9, anticorps S100a9
- Sujet
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Name/Gene ID: S100A9
Synonyms: S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14 - ID gène
- 6280
- NCBI Accession
- NP_002956
- UniProt
- P06702
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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