eIF4EBP1 anticorps (AA 101-118)
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- Antigène Voir toutes eIF4EBP1 (EIF4EBP1) Anticorps
- eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
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Épitope
- AA 101-118
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Reactivité
- Humain, Souris, Rat, Lapin, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp eIF4EBP1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
- Marque
- IHC-plus™
- Specificité
- Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).
- Purification
- Immunoaffinity purified
- Immunogène
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An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Top Product
- Discover our top product EIF4EBP1 Anticorps primaire
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- Indications d'application
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Approved: GS, IHC, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
- Conseil sur la manipulation
- avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
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- Antigène
- eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
- Autre désignation
- EIF4EBP1 / 4EBP1 (EIF4EBP1 Produits)
- Synonymes
- anticorps 4E-BP1, anticorps 4EBP1, anticorps BP-1, anticorps PHAS-I, anticorps 4e-bp1, anticorps AA959816, anticorps 153432_at, anticorps 43-BP, anticorps 4E-BP, anticorps 4EBP, anticorps 4e-bp, anticorps 4eBP, anticorps 4ebp, anticorps BcDNA.HL08053, anticorps BcDNA:HL08053, anticorps CG8846, anticorps CT8705, anticorps Dm 4E-BP, anticorps Dmel\\CG8846, anticorps Phas1, anticorps Thor/EIF4EBP, anticorps anon-WO0172774.158, anticorps d4E-BP, anticorps d4E-BP1, anticorps d4EBP, anticorps d4e-bp, anticorps d4eBP, anticorps dthor, anticorps eIF4E-BP, anticorps l(2)06270, anticorps l(2)k07736, anticorps l(2)k13517, anticorps pp20, anticorps thor, anticorps EIF4EBP1, anticorps fc04h09, anticorps wu:fc04h09, anticorps zgc:64137, anticorps eukaryotic translation initiation factor 4E binding protein 1, anticorps CG8846 gene product from transcript CG8846-RA, anticorps EIF4EBP1, anticorps Eif4ebp1, anticorps Thor, anticorps eif4ebp1
- Sujet
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Name/Gene ID: EIF4EBP1
Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1 - ID gène
- 1978
- UniProt
- Q13541
- Pathways
- Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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