PDGF-BB Homodimer anticorps
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- Antigène
- PDGF-BB Homodimer
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Human PDGF-BB
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Human PDGF-BB.
Type of Immunogen: Cell extract
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- Indications d'application
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Approved: ELISA, Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water to a concentration of 0.1-1.0 mg/mL
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- PDGF-BB Homodimer
- Autre désignation
- PDGF-BB
- Synonymes
- anticorps PDGF-2, anticorps PDGF2, anticorps SIS, anticorps SSV, anticorps c-sis, anticorps PDGF-B, anticorps Sis, anticorps platelet derived growth factor subunit B, anticorps platelet derived growth factor, B polypeptide, anticorps PDGFB, anticorps Pdgfb
- Sujet
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Name/Gene ID: PDGFB
Family: PDGF
Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain - ID gène
- 5155
- UniProt
- P01127
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