CNTF anticorps
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- Antigène Voir toutes CNTF Anticorps
- CNTF (Ciliary Neurotrophic Factor (CNTF))
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Reactivité
- Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CNTF est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Rat CNTF
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Rat CNTF.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CNTF Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 5 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Autre désignation
- CNTF (CNTF Produits)
- Synonymes
- anticorps GPA, anticorps CNTF, anticorps HCNTF, anticorps AI429687, anticorps ciliary neurotrophic factor, anticorps CNTF, anticorps Cntf
- Sujet
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Name/Gene ID: CNTF
Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF - ID gène
- 1270
- UniProt
- P26441
- Pathways
- Signalistation JAK/STAT
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