Oncostatin M anticorps
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- Antigène Voir toutes Oncostatin M (OSM) Anticorps
- Oncostatin M (OSM)
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Oncostatin M est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Human Oncostatin-M
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Human Oncostatin-M.
Type of Immunogen: Cell extract - Top Product
- Discover our top product OSM Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.18 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hOncostatin M (10 ng/mL), a concentration of 0.10-0.175 μg/mL of this antibody is required. ELISA: To detect hOncostatin M by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Oncostatin M (LS-C104706) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hOncostatin M. Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin M is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- Oncostatin M (OSM)
- Autre désignation
- OSM / Oncostatin M (OSM Produits)
- Synonymes
- anticorps OSM, anticorps OncoM, anticorps oncostatin M, anticorps OSM, anticorps Osm
- Sujet
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Name/Gene ID: OSM
Synonyms: OSM, Oncostatin-M, Oncostatin M - ID gène
- 5008
- UniProt
- P13725
- Pathways
- Signalistation JAK/STAT, Negative Regulation of Hormone Secretion
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