This antibody recognises the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EMR1. Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.
Réactivité croisée (Details)
Species reactivity (tested):Mouse.
Purification
Affinity Chromatography on Protein G
Immunogène
Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
RIA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. Thisproduct requires pre-treatment of paraffin sections (Proteinase K is recommended fortissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixedfor more than 24 hours).
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages. Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480