ELANE anticorps (AA 27-265)

N° du produit ABIN4886568

Détail du produit anti-ELANE anticorps

Antigène: ELANE (Elastase, Neutrophil Expressed (ELANE))

Épitope: AA 27-265

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ELANE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Neutrophil elastase(ELANE) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: elastase, neutrophil expressed
Protein Name: Neutrophil elastase

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265). Mouse Elastase/ELANE/ELA2 shares 92% amino acid (aa) sequence identity with rat Elastase/ELANE/ELA2.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur ELANE

Antigène: ELANE (Elastase, Neutrophil Expressed (ELANE))

Autre désignation: ELANE (ELANE Produits)

Synonymes: anticorps ELA2, anticorps GE, anticorps HLE, anticorps HNE, anticorps NE, anticorps PMN-E, anticorps SCN1, anticorps Ela2, anticorps F430011M15Rik, anticorps ela2, anticorps ELANE, anticorps elastase, neutrophil expressed, anticorps proteinase 3, anticorps ELANE, anticorps Elane, anticorps prtn3

Sujet: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Synonyms: ELA2 | ELANE | Elastase 2 | Elastase-2 | HLE | HNE | Leukocyte elastase | Medullasin | Neutrophil elastase | PMN E | PMN elastase | SCN1 | P08246

ID gène: 50701