PRAS40 anticorps (AA 227-256)

N° du produit ABIN3029887

Détail du produit anti-PRAS40 anticorps

Antigène: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Épitope: AA 227-256

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRAS40 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 227-256 from the human protein was used as the immunogen for this AKT1S1 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the AKT1S1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the AKT1S1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur PRAS40

Antigène: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Autre désignation: AKT1S1 (AKT1S1 Produits)

Synonymes: anticorps MGC81452, anticorps lobe, anticorps pras40, anticorps fb34a04, anticorps wu:fb34a04, anticorps Lobe, anticorps PRAS40, anticorps 1110012J22Rik, anticorps AI227026, anticorps Lobel, anticorps AKT1 substrate 1 (proline rich) S homeolog, anticorps AKT1 substrate 1, anticorps AKT1 substrate 1 (proline rich), anticorps AKT1 substrate 1 (proline-rich), anticorps akt1s1.S, anticorps AKT1S1, anticorps akt1s1, anticorps Akt1s1

Sujet: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. [UniProt]

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, L'effet Warburg