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Nestin anticorps (AA 1389-1416)

Name: Nestin anticorps (AA 1389-1416)
SKU: ABIN3031961
Price: 628.5 EUR
Availability: InStock

NES Reactivité: Humain WB, IHC, ELISA, FACS, IF Hôte: Lapin Polyclonal unconjugated

Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate (Nestin anticorps (AA 1389-1416))

IHC analysis of FFPE human hepatocarcinoma stained with Nestin antibody (Nestin anticorps (AA 1389-1416))

IHC

Western blot analysis of Nestin antibody and HepG2 lysate (Nestin anticorps (AA 1389-1416))

Flow cytometric analysis of HepG2 cells using Nestin antibody (bottom histogram) compared to a negative control cell (top histogram). (Nestin anticorps (AA 1389-1416))

FACS

Fluorescent confocal image of SY5Y cells stained with Nestin antibody at 1:200. (Nestin anticorps (AA 1389-1416))

Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate; Predicted band size : 170-220 kDa. (Nestin anticorps (AA 1389-1416))

8 images

N° du produit ABIN3031961

Fiche de données

Antigène Voir toutes Nestin (NES) Anticorps

Nestin (NES)
Épitope
16

16

15

10

8

7

6

5

5

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1389-1416
Reactivité
138

74

55

4

3

2

2

2

1

1

1

1
Humain
Hôte
108

40

3

1

1
Lapin
Anticorps secondaires appropriés
Clonalité
113

40
Polyclonal
Conjugué
64

10

9

7

7

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3
Cet anticorp Nestin est non-conjugé
Application
75

49

48

47

41

39

35

27

25

16

10

6

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purification

Antigen affinity purified

Immunogène

A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.

Isotype

Ig Fraction

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NES Reactivité: Souris IHC, ICC Hôte: Rat Monoclonal 7A3 unconjugated

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NES Reactivité: Humain IF, ICC Hôte: Souris Monoclonal 10C2 FITC

anti-Nestin (NES) (AA 419-588) antibody
NES Reactivité: Humain WB, ELISA, FACS Hôte: Souris Monoclonal 1A9G12 unconjugated

Indications d'application

Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

Aliquot the Nestin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Antigène

Nestin (NES)

Autre désignation

Nestin (NES Produits)

Synonymes

anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes

Sujet

Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

UniProt

P48681