Suitable for use in Western blotting, and Immunocytochemistry/Immunofluorescence. Recommended Dilutions: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
0.1 M Tris, 0.1 M Glycine, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at -20 °C.
anticorps MGC64314, anticorps zeh1094, anticorps zgc:85649, anticorps hm:zeh1094, anticorps wu:fa96a09, anticorps wu:fj39g07, anticorps HTF9A, anticorps Htf9a, anticorps RAN binding protein 1 S homeolog, anticorps RAN binding protein 1 L homeolog, anticorps ran binding protein 1, anticorps RAN binding protein 1, anticorps ran-specific GTPase-activating protein pseudogene, anticorps ranbp1.S, anticorps ranbp1.L, anticorps PVX_090070, anticorps GL50803_16969, anticorps LOC100343953, anticorps ranbp1, anticorps RANBP1, anticorps Ranbp1
Sujet
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92 % similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.Synonyms: Ran-binding protein 1, Ran-specific GTPase-activating protein