DGKE anticorps
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- Antigène Voir toutes DGKE Anticorps
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DGKE est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of DGK-ε protein. (region surrounding Ser194)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Pureté
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product DGKE Anticorps primaire
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- Indications d'application
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Antigène
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
- Autre désignation
- DAG Kinase epsilon (DGKE Produits)
- Synonymes
- anticorps MGC81643, anticorps DGKE, anticorps C87606, anticorps DAGK6, anticorps DGK, anticorps DAGK5, anticorps NPHS7, anticorps diacylglycerol kinase epsilon S homeolog, anticorps diacylglycerol kinase epsilon, anticorps diacylglycerol kinase, epsilon, anticorps dgke.S, anticorps DGKE, anticorps dgke, anticorps LOC5575244, anticorps Dgke
- Sujet
- Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DAGK5, DGK-epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon
- Poids moléculaire
- approx. 60 kDa
- ID gène
- 8526
- NCBI Accession
- NP_003638
- UniProt
- P52429
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