This antibody detects Decorin (Internal). It is expected to recognize the three reported isoforms a, b and c (NP_001911.1, NP_598011.1, NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.
Réactivité croisée (Details)
Species reactivity (expected):Cow, pig, caviaSpecies reactivity (tested):Human
Purification
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogène
Peptide from the internal region of the protein sequence according to NP_001911.1, NP_598011.1, NP_598012.1
Peptide ELISA: limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 38 kDa band observed in Human Kidney lysates(calculated MW of 39.8 kDa according to NP_001911.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.
Decorin is a member of the small, leucine rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF beta. It is believed that decorin also plays a role in the cell cycle of chondrocytes.Synonyms: Bone proteoglycan II, DCN, PG-S2, PG40, SLRR1B