AES anticorps (C-Term, N-Term)

N° du produit ABIN499225

Détail du produit anti-AES anticorps

Antigène: AES (Amino-terminal Enhancer of Split (AES))

Épitope: C-Term, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AES est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificité: AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.

Purification: Affinity chromatography purified via peptide column

Immunogène: Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: AES antibody can be used for the detection of AES at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur AES

Antigène: AES (Amino-terminal Enhancer of Split (AES))

Autre désignation: AES / ESP1 (AES Produits)

Synonymes: anticorps AES-1, anticorps AES-2, anticorps ESP1, anticorps GRG, anticorps GRG5, anticorps TLE5, anticorps AL024115, anticorps Esp1, anticorps Grg, anticorps Grg-5, anticorps Grg5, anticorps Tle5, anticorps zgc:64216, anticorps aes-1, anticorps aes-2, anticorps esp1, anticorps grg, anticorps grg5, anticorps tle5, anticorps xgrg-5, anticorps AES, anticorps amino-terminal enhancer of split, anticorps amino-terminal enhancer of split S homeolog, anticorps AES, anticorps Aes, anticorps aes, anticorps aes.S

Sujet: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM

ID gène: 166

NCBI Accession: NP_001121

UniProt: Q08117

Pathways: Signalisation WNT, SARS-CoV-2 Protein Interactome