Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
Specificité
Caspase-9 antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9. It has no cross response to other members in caspase family.
Purification
Affinity chromatography purified via peptide column
CASP9
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA. Western Blot: Caspase-9 antibody can be used for detection of caspase-9 at 1/1000 to1/2000 dilution. A 46 kDa band should be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6