ELISA. Western blot: 0.5 - 2 μg/mL, a band at approximately 32 kDa can be detected. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 0.02 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Antigène
PHAP1 (ANP32A)
(Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))
anticorps C15orf1, anticorps HPPCn, anticorps I1PP2A, anticorps LANP, anticorps MAPM, anticorps PHAP1, anticorps PHAPI, anticorps PP32, anticorps Anp32, anticorps W91701, anticorps pp32, anticorps Anp32a, anticorps CG5784, anticorps CT18148, anticorps CT42180, anticorps Dmel\\CG5784, anticorps anon-EST:fe3A2, anticorps wu:fj36e08, anticorps zgc:55516, anticorps ANP32D, anticorps acidic nuclear phosphoprotein 32 family member A, anticorps acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, anticorps CG5784 gene product from transcript CG5784-RB, anticorps acidic nuclear phosphoprotein 32 family member A L homeolog, anticorps ANP32A, anticorps Anp32a, anticorps Mapmodulin, anticorps anp32a, anticorps anp32a.L
Sujet
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.Synonyms: Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, C15orf1, LANP, Leucine-rich acidic nuclear protein, MAPM, Mapmodulin, PHAP1, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I