PUMA anticorps (N-Term)
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- Antigène Voir toutes PUMA (BBC3) Anticorps
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Épitope
- N-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PUMA est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Specificité
- This antibody detects PUMA at N-term.
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Peptide Affinity Chromatography
- Immunogène
- Synthetic peptide corresponding to 14 amino acids near the amino terminus of Human PUMA-alpha. .Remarks: This sequence is identical between alpha and beta forms of the PUMA proteins.
- Isotype
- IgG
- Top Product
- Discover our top product BBC3 Anticorps primaire
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- Indications d'application
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ELISA. Western blot: 2 to 4 μg/mL, K562 cell lysate can be used as a positive control and a band atapproximately 23 kDa can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- PBS, 0.02 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Autre désignation
- PUMA (BBC3 Produits)
- Synonymes
- anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3
- Sujet
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
- ID gène
- 27113
- NCBI Accession
- NP_055232
- UniProt
- Q9BXH1
- Pathways
- Signalisation p53, Positive Regulation of Endopeptidase Activity
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