RNF168 anticorps (C-Term)

N° du produit ABIN500624

Détail du produit anti-RNF168 anticorps

Antigène: RNF168 (Ring Finger Protein 168 (RNF168))

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF168 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody detects RNF168.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogène: RNF168 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human RNF168.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-RNF168 (ABIN500624)

Li, Guan, Huang, Hu, Zheng: "RNF168-mediated H2A neddylation antagonizes ubiquitylation of H2A and regulates DNA damage repair." dans: Journal of cell science, Vol. 127, Issue Pt 10, pp. 2238-48, (2014) (PubMed).

Détails sur RNF168

Antigène: RNF168 (Ring Finger Protein 168 (RNF168))

Autre désignation: RNF168 (RNF168 Produits)

Synonymes: anticorps 3110001H15Rik, anticorps hRNF168, anticorps ring finger protein 168, anticorps ring finger protein 168, E3 ubiquitin protein ligase L homeolog, anticorps Rnf168, anticorps rnf168.L, anticorps RNF168

Sujet: RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.Synonyms: E3 ubiquitin-protein ligase RNF168, RING finger protein 168

ID gène: 165918

NCBI Accession: NP_689830

UniProt: Q8IYW5

Pathways: Production of Molecular Mediator of Immune Response