STIM1
Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache), Cobaye, Cheval
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA. Western blot: 1 - 2 μg/mL. Immunohisochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1,0 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Wu, Hsu, Wang, Liang, Tsai, Yen, Li, Chiu, Chang, Huang, Lin, Yoshioka: "A Novel Strategy for TNF-Alpha Production by 2-APB Induced Downregulated SOCE and Upregulated HSP70 in O. tsutsugamushi-Infected Human Macrophages." dans: PLoS ONE, Vol. 11, Issue 7, pp. e0159299, (2017) (PubMed).
Misceo, Holmgren, Louch, Holme, Mizobuchi, Morales, De Paula, Stray-Pedersen, Lyle, Dalhus, Christensen, Stormorken, Tjønnfjord, Frengen: "A dominant STIM1 mutation causes Stormorken syndrome." dans: Human mutation, Vol. 35, Issue 5, pp. 556-64, (2014) (PubMed).
In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form.Synonyms: Stromal interaction molecule 1