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MFN1 anticorps (AA 1-234)

MFN1 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN5013951
  • Antigène Voir toutes MFN1 Anticorps
    MFN1 (Mitofusin 1 (MFN1))
    Épitope
    • 16
    • 7
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-234
    Reactivité
    • 51
    • 30
    • 27
    • 21
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 61
    • 5
    • 3
    • 1
    Lapin
    Clonalité
    • 58
    • 12
    Polyclonal
    Conjugué
    • 33
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MFN1 est non-conjugé
    Application
    • 53
    • 26
    • 15
    • 11
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Fonction
    Polyclonal Antibody to Mitofusin 1 (MFN1)
    Specificité
    The antibody is a rabbit polyclonal antibody raised against MFN1. It has been selected for its ability to recognize MFN1 in immunohistochemical staining and western blotting.
     Réactivité croisée
    Humain, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogène
    Recombinant Mitofusin 1 (MFN1) corresdonding to Met1~Ile234
    Isotype
    IgG
    Top Product
    Discover our top product MFN1 Anticorps primaire
  • Indications d'application

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Validation #103013 (Western Blotting)
    'Independent Validation' signe
    by
    Wang Lab, Department of Animal Science, College of Agriculture & Natural Resources
    No.
    #103013
    Date
    30.05.2018
    Antigène
    MFN1
    Numéro du lot
    A20180411903
    Application validée
    Western Blotting
    Contrôle positif

    293T cells transfected with MFN1-HA expression vector; the full length MFN1 CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

    Contrôle négative

    293T cells transfected with an LUC-HA expression vector, and the full length LUC CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

    293T cells transfected with MFN2-HA expression vector; the full length MFN2 CDS has been inserted upstream of an FLAG-tag in plasmid pcDNA3.1.

    Conclusion

    Passed. ABIN5013951 specifically recognizes antigen in sample.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN5013951
    Anticorps secondaire
    goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515)
    Full Protocol
    • Grow 293T cells in DMEM medium (Gibco, lot 1897400) supplemented with 10% fetal bovine serum (Gibco, lot 1913756) and 1% Penicillin-Streptomycin (thermo, 10378016), at 37°C and 7% CO2 in 6-well plates to 60-70% confluency.
    • Transfect cells with plasmids expressing either MFN1-HA, MFN2-HA or LUC-HA with max 2µg of plasmid using PEI transfection reagent (Poly-Science, 24765-1) following the manufacturer´s instructions.
    • Lyse cells in 100µl RIPA buffer containing protease inhibitors (Sigma-Aldrich, P8340).
    • Determine total protein content of the lysates using a Bradford protein assay (Protein Assay Dye Reagent Concentrate, Bio-Rad, 5000006).
    • Denature 100µg total protein for 10min at 95°C in 1x SDS loading buffer and subsequently separate them on a freshly cast 10% denaturing polyacrylamide gel.
    • Transfer proteins onto Immun-Blot Low Fluorescence PVDF Membrane (0.45μm) at 1.0A and up to 25V for 30min via Trans-Blot Turbo Transfer System.
    • Block the membrane with Bio-rad Blotting-Grade Blocker (5% non-fat milk in PBS).
    • Incubation with primary
      • rabbit anti-MFN1 antibody (antibodies-online, ABIN5013951, lot A20180411903) diluted 1:200 in Blocking Buffer (5% non-fat milk) ON at 4°C.
      • mouse anti-HA-tag antibody (Santa Cruz, sc-7392) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C (only LUC-HA expressing cells).
      • mouse anti-MFN2-tag antibody (Santa Cruz, sc-100560) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C.
      • mouse anti-beta-actin antibody (Sigma-Aldrich, A1978) diluted 1:10000 in Blocking Buffer(5% non-fat milk) ON at 4°C.
    • Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
    • Incubation with secondary goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515) or goat anti-mouse IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6516) diluted 1:10000 in Odyssey Blocking Buffer for 1h at RT.
    • Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
    • Add Clarity and Clarity Max Western ECL Blotting Substrates and scan the membrane on a Bio-Rad ChemiDoc XRS+System.
    Notes

    The antigen antibody ABIN5013951 reveals a protein of the expected molecular weight of antigen in lysates of cells. The protein bands is only visible in the positive but not the negative controls.

  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Agent conservateur
    ProClin
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Date de péremption
    24 months
  • Antigène
    MFN1 (Mitofusin 1 (MFN1))
    Autre désignation
    Mitofusin 1 (MFN1 Produits)
    Synonymes
    anticorps mfn1, anticorps zgc:66150, anticorps mitofusin-1, anticorps MFN1, anticorps MFN2, anticorps hfzo1, anticorps hfzo2, anticorps 2310002F04Rik, anticorps 6330416C07Rik, anticorps D3Ertd265e, anticorps HR2, anticorps mKIAA4032, anticorps Fzo1b, anticorps mitofusin 1b, anticorps mitofusin 1, anticorps mitofusin 1 S homeolog, anticorps mfn1b, anticorps MFN1, anticorps mfn1.S, anticorps mfn1, anticorps Mfn1
    Sujet
    Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog
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