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Acrolein anticorps (Atto 390)

ACR ELISA, FACS, WB, ICC, IF, IHC Hôte: Souris Monoclonal 10A10 Atto 390
N° du produit ABIN5067182
  • Antigène Tous les produits Acrolein (ACR)
    Acrolein (ACR)
    Hôte
    • 9
    Souris
    Clonalité
    • 9
    Monoclonal
    Conjugué
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Acrolein est conjugé à/à la Atto 390
    Application
    ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Specificité
    Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
    Purification
    Protein G Purified
    Immunogène
    Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
    Clone
    10A10
    Isotype
    IgG1
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN5067182 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Acrolein (ACR)
    Autre désignation
    Acrolein (ACR Produits)
    Classe de substances
    Chemical
    Sujet
    Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
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