HNE anticorps
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- Antigène Voir toutes HNE Anticorps
- HNE (4-Hydroxynonenal (HNE))
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HNE est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificité
- Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
- Purification
- Protein G Purified
- Immunogène
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- Clone
- 12F7
- Isotype
- IgG1
- Top Product
- Discover our top product HNE Anticorps primaire
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- Indications d'application
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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A 1:1000 dilution of ABIN5067289 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- -20°C
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- Antigène
- HNE (4-Hydroxynonenal (HNE))
- Autre désignation
- 4-Hydroxynonenal (HNE Produits)
- Classe de substances
- Chemical
- Sujet
- 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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