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HNE anticorps (Biotin)

HNE WB, ELISA, ICC, IF Hôte: Souris Monoclonal 12F7 Biotin
N° du produit ABIN5067300
  • Antigène Voir toutes HNE Anticorps
    HNE (4-Hydroxynonenal (HNE))
    Hôte
    • 15
    • 11
    • 1
    Souris
    Clonalité
    • 16
    • 11
    Monoclonal
    Conjugué
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HNE est conjugé à/à la Biotin
    Application
    • 26
    • 13
    • 13
    • 9
    • 9
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificité
    Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
    Purification
    Protein G Purified
    Immunogène
    Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
    Clone
    12F7
    Isotype
    IgG1
    Top Product
    Discover our top product HNE Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN5067300 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    HNE (4-Hydroxynonenal (HNE))
    Autre désignation
    4-Hydroxynonenal (HNE Produits)
    Classe de substances
    Chemical
    Sujet
    4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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