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Sulfotyrosine anticorps (Atto 390)

WB, ELISA, ICC, IF, IHC Hôte: Souris Monoclonal 7C5 Atto 390
N° du produit ABIN5067488
  • Antigène Tous les produits Sulfotyrosine
    Sulfotyrosine
    Hôte
    • 10
    Souris
    Clonalité
    • 10
    Monoclonal
    Conjugué
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Sulfotyrosine est conjugé à/à la Atto 390
    Application
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Specificité
    Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
    Purification
    Protein G Purified
    Immunogène
    Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    Clone
    7C5
    Isotype
    IgG1
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN5067488 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Sulfotyrosine
    Abstract
    Sulfotyrosine Produits
    Classe de substances
    Amino Acid
    Sujet
    Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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