Sulfotyrosine anticorps (HRP)

N° du produit ABIN5067500

Détail du produit anti-Sulfotyrosine anticorps (HRP)

Antigène: Sulfotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Sulfotyrosine est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificité: Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Purification: Protein G Purified

Immunogène: Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Clone: 7C5

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN5067500 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Sulfotyrosine

Antigène: Sulfotyrosine

Abstract: Sulfotyrosine Produits

Classe de substances: Amino Acid

Sujet: Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.