Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Specificité
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
KIR2DL4
Reactivité: Humain, Souris, Rat
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow cytometry: Recommended dilution: 2-6 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Rajagopalan, Bryceson, Kuppusamy, Geraghty, van der Meer, Joosten, Long: "Activation of NK cells by an endocytosed receptor for soluble HLA-G." dans: PLoS biology, Vol. 4, Issue 1, pp. e9, (2006) (PubMed).
LeMaoult, Zafaranloo, Le Danff, Carosella: "HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 662-4, (2005) (PubMed).
Yan, Fan: "Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition." dans: Cell research, Vol. 15, Issue 3, pp. 176-82, (2005) (PubMed).
Goodridge, Witt, Christiansen, Warren: "KIR2DL4 (CD158d) genotype influences expression and function in NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1768-74, (2003) (PubMed).
Rajagopalan, Fu, Long: "Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 1877-81, (2001) (PubMed).
Antigène
KIR2DL4/CD158d (KIR2DL4)
(Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 4 (KIR2DL4))
anticorps G9P, anticorps CD158D, anticorps KIR103, anticorps KIR103AS, anticorps KIR2DL4, anticorps killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4, anticorps killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4, anticorps KIR2DL4
Sujet
Killer cell immunoglobulin like receptor, two Ig d,CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ, secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.,KIR2DL4, KIR103AS, 103AS, 15.212