COX1 anticorps (C-Term)
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- Antigène Voir toutes COX1 Anticorps
- COX1 (Cytochrome C Oxidase Subunit I (COX1))
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Épitope
- AA 501-514, C-Term
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Reactivité
- Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp COX1 est non-conjugé
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB, IHC-P, ICC in Mouse,Rat.
- Séquence
- PYHTFEEPTY VKVK
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
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Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1(MT-CO1) detection. Tested with WB, IHC-P, ICC in Mouse,Rat.
Gene Name: cytochrome c oxidase I, mitochondrial
Protein Name: Cytochrome c oxidase subunit 1 - Purification
- Immunogen affinity purified.
- Immunogène
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse MTCO1(501-514aa PYHTFEEPTYVKVK), different from the related rat sequence by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product COX1 Anticorps primaire
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- Indications d'application
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Agent conservateur
- Sodium azide, Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Deletion of mitochondrial anchoring protects dysmyelinating shiverer: implications for progressive MS." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 13, pp. 5293-306, (2015) (PubMed).
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Deletion of mitochondrial anchoring protects dysmyelinating shiverer: implications for progressive MS." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 13, pp. 5293-306, (2015) (PubMed).
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- Antigène
- COX1 (Cytochrome C Oxidase Subunit I (COX1))
- Autre désignation
- mt-Co1 (COX1 Produits)
- Synonymes
- anticorps COX-1, anticorps COI, anticorps MTCO1, anticorps COX1, anticorps CoxI, anticorps prostaglandin-endoperoxide synthase 1, anticorps cytochrome c oxidase subunit I, anticorps cytochrome c oxidase subunit 1, anticorps cytochrome c oxidase 1, anticorps PTGS1, anticorps COX1, anticorps cox1
- Sujet
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Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
Synonyms: COI antibody|COX I antibody|COX1_HUMAN antibody|COXI antibody|Cytochrome c oxidase polypeptide I antibody|Cytochrome c oxidase subunit 1 antibody|Cytochrome C Oxidase subunit I antibody|Mitochondrially encoded cytochrome c oxidase I antibody|MT CO1 antibody|MT-CO1 antibody|MTCO 1 antibody|MTCO1 antibody - UniProt
- P00397
- Pathways
- Proton Transport
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