GAPDH anticorps (AA 136-335)

N° du produit ABIN5518757

Détail du produit anti-GAPDH anticorps

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Épitope: AA 136-335

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glyceraldehyde-3-phosphate dehydrogenase
Protein Name: Glyceraldehyde-3-phosphate dehydrogenase

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-GAPDH (ABIN5518757)

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Li, Guan, Liu, Zhao, Li, Zhang, Tan, Mi, Li: "Epoxyeicosanoids prevent intervertebral disc degeneration in vitro and in vivo." dans: Oncotarget, Vol. 8, Issue 3, pp. 3781-3797, (2018) (PubMed).

Hu, Cao, Pang, Luo, Zou, Feng, Li, Chen, Huang: "Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression." dans: Redox biology, Vol. 12, pp. 682-689, (2018) (PubMed).

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Cai, Tan, Li, Gao, Li, Li, Tang, Li, Hui: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).

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Liu, Liu, Tan, Tang, Long, Li, Liang, Chu, Yang: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." dans: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).

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Zou, Zhang, Yuan, Yang, Wu, Song, Zhu, Mao, Chen: "Endogenous hormone 2-methoxyestradiol suppresses venous hypertension-induced angiogenesis through up- and down-regulating p53 and id-1." dans: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 957-967, (2018) (PubMed).

Huang, Pan, Mao, Zhang, Ma, Xi, You: "Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes." dans: Inflammation, Vol. 41, Issue 2, pp. 562-568, (2018) (PubMed).

Miao, Margolis, Gao, Voss, Li, Martini, Norton, Bossé, Wankowicz, Cullen, Horak, Wind-Rotolo, Tracy, Giannakis, Hodi, Drake, Ball, Allaf, Snyder, Hellmann, Ho, Motzer, Signoretti, Kaelin, Choueiri et al.: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." dans: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." dans: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH (GAPDH Produits)

Synonymes: anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985

Sujet: Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Synonyms: EC 1.2.1.12 | EC1.2.1.12 | G3P | GAPD | GAPDH | P04406

ID gène: 2597

UniProt: P04406