Choline Acetyltransferase anticorps (AA 446-652)

N° du produit ABIN5518818

Détail du produit anti-Choline Acetyltransferase anticorps

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Épitope: AA 446-652

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Choline O-acetyltransferase(CHAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Choline O-acetyltransferase(CHAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: choline O-acetyltransferase
Protein Name: Choline O-acetyltransferase

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Choline Acetyltransferase (ABIN5518818)

Yu, Xia, Tang, Xu, Wei, Chen, Dai, Gong, Bi: "Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation." dans: Scientific reports, Vol. 7, pp. 40802, (2018) (PubMed).

Zhao, Xu, Pan, Zhu, Fu, Guo, Lu, Wang: "Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse model of chronic stress." dans: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 234, (2018) (PubMed).

Hu, Wang, Ren, Liu: "Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2." dans: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 200, (2017) (PubMed).

Wang, He, Zhong, Lv, Xiao, Liu: "Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons." dans: BioMed research international, Vol. 2015, pp. 857202, (2016) (PubMed).

Liu, Deng, Zhang, Zhang: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." dans: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).

Zhang, Li, Gu, Liu, Xu, Cui, Sun: "Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma." dans: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 60, (2009) (PubMed).

Zhang, Qi, Li, Zhang, Xu, Wang, Sun: "Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer." dans: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 62, (2009) (PubMed).

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: CHAT (CHAT Produits)

Synonymes: anticorps CHOACTASE, anticorps CMS1A, anticorps CMS1A2, anticorps ChoAcTase, anticorps B230380D24Rik, anticorps choline O-acetyltransferase, anticorps choline O-acetyltransferase a, anticorps choline acetyltransferase, anticorps carnitine O-acetyltransferase, anticorps CHAT, anticorps chata, anticorps Chat, anticorps PGTG_00551, anticorps LOC100533322

Sujet: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Synonyms: ChAT | CHOACTase | Choline acetylase | choline acetyltransferase | Choline O acetyltransferase | CMS1A | CMS1A2 | CMS6 | P28329

ID gène: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development