RAN anticorps (AA 2-216)

N° du produit ABIN5518950

Détail du produit anti-RAN anticorps

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Épitope: AA 2-216

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for GTP-binding nuclear protein Ran(RAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for GTP-binding nuclear protein Ran(RAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: RAN, member RAS oncogene family
Protein Name: GTP-binding nuclear protein Ran

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur RAN

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Autre désignation: RAN (RAN Produits)

Synonymes: anticorps ARA24, anticorps Gsp1, anticorps TC4, anticorps ran, anticorps ara24, anticorps gsp1, anticorps ran-1, anticorps tc4, anticorps RAN, anticorps RANP1, anticorps AAF30287, anticorps CG1404, anticorps Dmel\CG1404, anticorps Ran, anticorps dran, anticorps l(1)G0075, anticorps ran10A, anticorps fc16b04, anticorps wu:fc16b04, anticorps RAN, member RAS oncogene family, anticorps RAN, member RAS oncogene family S homeolog, anticorps CG1404 gene product from transcript CG1404-RC, anticorps RAN, anticorps Ran, anticorps ran.S, anticorps ran

Sujet: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Synonyms: GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24, OK/SW-cl.81

ID gène: 5901

UniProt: P62826

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus