STAT5A anticorps (AA 681-692)

N° du produit ABIN5539582

Détail du produit anti-STAT5A anticorps

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Épitope: AA 681-692

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT5A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Fonction: STAT5A (aa681-692)

Séquence: KYYTPVLAKA VD

Specificité: This antibody is not expected to cross-react with STAT5B.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line K562 (calculated MW of 90.6 kDa according to NP_003143.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur STAT5A

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Autre désignation: STAT5A (STAT5A Produits)

Synonymes: anticorps MGF, anticorps STAT5, anticorps AA959963, anticorps Stat5, anticorps STAT5A/MGF, anticorps STATA5, anticorps STAT5A, anticorps stat5, anticorps stat5b, anticorps signal transducer and activator of transcription 5A, anticorps signal transducer and activator of transcription 5a, anticorps STAT5A, anticorps Stat5a, anticorps stat5a, anticorps LOC100348518, anticorps LOC100716811

Sujet: STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5

ID gène: 6776, 20850, 24918

NCBI Accession: NP_003143

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation