DYRK1A anticorps (N-Term)

N° du produit ABIN5539713

Détail du produit anti-DYRK1A anticorps

Antigène: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DYRK1A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Fonction: DYRK1A

Séquence: PHSHQYSDRR QPN

Specificité: This antibody is expected to recognize all reported isoforms (NP_001387.2, NP_569120.1, NP_567824.1, NP_569122.1). This antibody is not expected to cross-react with DYRK1B.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 80 kDa band observed in lysates of cell line K562 (calculated MW of 85.6 kDa according to NP_001387.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15,11(1):297. PMID: 29764512.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-DYRK1A (ABIN5539713)

Widowati, Bamberg-Lemper, Becker: "Mutational analysis of two residues in the DYRK homology box of the protein kinase DYRK1A." dans: BMC research notes, Vol. 11, Issue 1, pp. 297, (2018) (PubMed).

Widowati, Ernst, Hausmann, Müller-Newen, Becker: "Functional characterization of DYRK1A missense variants associated with a syndromic form of intellectual deficiency and autism." dans: Biology open, Vol. 7, Issue 4, (2018) (PubMed).

Détails sur DYRK1A

Antigène: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Autre désignation: DYRK1A (DYRK1A Produits)

Synonymes: anticorps DYRK1A, anticorps dyrk, anticorps dyrk1, anticorps hp86, anticorps mnb, anticorps mnbh, anticorps DYRK, anticorps DYRK1, anticorps HP86, anticorps MNB, anticorps MNBH, anticorps MRD7, anticorps 2310043O08Rik, anticorps D16Ertd272e, anticorps D16Ertd493e, anticorps Dyrk, anticorps ENSMUSG00000074897, anticorps Gm10783, anticorps Mnbh, anticorps Mp86, anticorps mmb, anticorps PSK47, anticorps dual specificity tyrosine phosphorylation regulated kinase 1A, anticorps dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, anticorps dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a, anticorps dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A S homeolog, anticorps DYRK1A, anticorps dyrk1a, anticorps Dyrk1a, anticorps dyrk1a.S

Sujet: DYRK1A, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, DYRK, DYRK1, HP86, MNB, MNBH, MRD7, MNB/DYRK protein kinase, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, dual specificity YAK1-related kinase, d

ID gène: 1859, 13548, 25255

NCBI Accession: NP_001387, NP_569120, NP_567824, NP_569122

Pathways: Mitotic G1-G1/S Phases