DYRK1A anticorps (N-Term)

N° du produit ABIN5539713

Détail du produit anti-DYRK1A anticorps

Antigène: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DYRK1A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Fonction: DYRK1A

Séquence: PHSHQYSDRR QPN

Specificité: This antibody is expected to recognize all reported isoforms (NP_001387.2, NP_569120.1, NP_567824.1, NP_569122.1). This antibody is not expected to cross-react with DYRK1B.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 80 kDa band observed in lysates of cell line K562 (calculated MW of 85.6 kDa according to NP_001387.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15,11(1):297. PMID: 29764512.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-DYRK1A (ABIN5539713)

Widowati, Bamberg-Lemper, Becker: "Mutational analysis of two residues in the DYRK homology box of the protein kinase DYRK1A." dans: BMC research notes, Vol. 11, Issue 1, pp. 297, (2018) (PubMed).

Widowati, Ernst, Hausmann, Müller-Newen, Becker: "Functional characterization of DYRK1A missense variants associated with a syndromic form of intellectual deficiency and autism." dans: Biology open, Vol. 7, Issue 4, (2018) (PubMed).

Détails sur DYRK1A

Antigène: DYRK1A (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 1A (DYRK1A))

Autre désignation: DYRK1A (DYRK1A Produits)

Sujet: DYRK1A, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, DYRK, DYRK1, HP86, MNB, MNBH, MRD7, MNB/DYRK protein kinase, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, dual specificity YAK1-related kinase, d

ID gène: 1859, 13548, 25255

NCBI Accession: NP_001387, NP_569120, NP_567824, NP_569122

Pathways: Mitotic G1-G1/S Phases