CEACAM5/6 anticorps

N° du produit ABIN5541175

Détail du produit anti-CEACAM5/6 anticorps

Antigène: CEACAM5/6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CEACAM5/6 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: This antibody reacts to CD66c/e. Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogène: MUS was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).

Clone: MUS

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western blot: 4 μg/mL. Immunohistochemistry: 1-2 μg/10 cells (on Cryosections).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-CEACAM5/6 (ABIN5541175)

Voges, Bachmann, Naujoks, Kopp, Hauck: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." dans: PLoS ONE, Vol. 7, Issue 6, pp. e39908, (2012) (PubMed).

Tsutsumida, Swanson, Singh, Caffrey, Kitajima, Goto, Yonezawa, Hollingsworth: "RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 10, pp. 2976-87, (2006) (PubMed).

Détails sur CEACAM5/6

Antigène: CEACAM5/6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))

Autre désignation: cd66c/e (CEACAM5/6 Produits)

Synonymes: anticorps CEACAM5, anticorps CEA, anticorps carcinoembryonic antigen related cell adhesion molecule 5, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps CEACAM5

Sujet: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly upregulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).