This antibody detects Invertase [Candida]. Cross reactivity against Invertase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Invertase [Candida].
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western blot : 1/500-1/2,500. ELISA : 1/1,000-1/5,000. This product has been assayed against 1.0 μg of Invertase [Candida] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested.
Restrictions
For Research Use only
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.