Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.2 without preservatives
Agent conservateur
Without preservative
Stock
4 °C,-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Krause, Behrends, Borowski, Lohrmann, Lang, Myrtek, Lorenzen, Virchow, Luttmann, Friedrich: "Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1." dans: Molecular immunology, Vol. 43, Issue 11, pp. 1799-807, (2006) (PubMed).
Bratke, Kuepper, Bade, Virchow, Luttmann: "Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood." dans: European journal of immunology, Vol. 35, Issue 9, pp. 2608-16, (2005) (PubMed).
Antigène
GZMK
(Granzyme K (Granzyme 3, Tryptase II) (GZMK))
Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.