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Cyclin D1 anticorps (AA 167-295)

Name: Cyclin D1 anticorps (AA 167-295)
Brand: antibodies-online
SKU: ABIN5542302
Price: 614.71 EUR
Availability: InStock

CCND1 Reactivité: Humain ELISA, IHC, FACS, ICC Hôte: Souris Monoclonal 3B6G3 unconjugated

Western blot analysis using CCND1 mAb against human CCND1 (AA: 167-295) recombinant protein. (Cyclin D1 anticorps (AA 167-295))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Cyclin D1 anticorps (AA 167-295))

ELISA

Western blot analysis using CCND1 mAb against HEK293 (1) and CCND1 (AA: 167-295)-hIgGFc transfected HEK293 (2) cell lysate. (Cyclin D1 anticorps (AA 167-295))

Flow cytometric analysis of MCF-7 cells using CCND1 mouse mAb (green) and negative control (red). (Cyclin D1 anticorps (AA 167-295))

FACS

Immunofluorescence analysis of Hela cells using CCND1 mouse mAb (green). (Cyclin D1 anticorps (AA 167-295))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCND1 mouse mAb with DAB staining. (Cyclin D1 anticorps (AA 167-295))

IHC

6 images

N° du produit ABIN5542302

Fiche de données

Antigène Voir toutes Cyclin D1 (CCND1) Anticorps

Cyclin D1 (CCND1)
Épitope
22

21

16

16

12

11

9

9

8

7

7

7

6

6

5

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 167-295
Reactivité
235

126

121

40

20

18

16

12

10

9

3

3

3

3

3

2

2

1

1

1

1

1

1
Humain
Hôte
203

54

1
Souris
Anticorps secondaires appropriés
Clonalité
193

63
Monoclonal
Conjugué
118

25

20

9

7

5

5

5

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
Cet anticorp Cyclin D1 est non-conjugé
Application
196

86

64

61

41

30

28

27

23

20

18

12

6

5

3

2

2

2

1

1

1

1
ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Fonction

CCND1 Antibody

Purification

Purified antibody

Immunogène

Purified recombinant fragment of human CCND1 (AA: 167-295) expressed in E. Coli.

Clone

3B6G3

Isotype

IgG1

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CCND1 Reactivité: Rat WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/500

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Antigène

Cyclin D1 (CCND1)

Autre désignation

CCND1 (CCND1 Produits)

Synonymes

anticorps BCL1, anticorps D11S287E, anticorps PRAD1, anticorps U21B31, anticorps AI327039, anticorps CycD1, anticorps Cyl-1, anticorps bcl-1, anticorps cD1, anticorps cb161, anticorps cycd1, anticorps etID37810.7, anticorps fb52e01, anticorps fc45c08, anticorps fc83a12, anticorps id:ibd1198, anticorps wu:fb52e01, anticorps wu:fc45c08, anticorps wu:fc83a12, anticorps ccnd1b, anticorps prad1, anticorps ccnd1, anticorps ccnd1a, anticorps bcl1, anticorps d11s287e, anticorps u21b31, anticorps CCND1, anticorps cyclin D1, anticorps cyclin D1 S homeolog, anticorps cyclin D1 L homeolog, anticorps CCND1, anticorps Ccnd1, anticorps ccnd1, anticorps ccnd1.S, anticorps ccnd1.L

Sujet

Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Aliases: BCL1, PRAD1, U21B31, D11S287E

Poids moléculaire

33.7kDa

ID gène

595

HGNC

712

UniProt

P24385

Pathways

Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling