Alkaline Phosphatase anticorps (HRP)

N° du produit ABIN5596688

Détail du produit anti-Alkaline Phosphatase anticorps (HRP)

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): No cross reactivity ocurs against mammalian forms of Alkaline Phosphatase.

Purification: Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli].

Immunogène:

Immunogen: Alkaline Phosphatase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anit-alkaline phosphatase has been assayed against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase (ALP Produits)

Synonymes: anticorps ALP, anticorps ALP1, anticorps CRASH, anticorps ECK0378, anticorps JW0374, anticorps psiA, anticorps 2410004D18Rik, anticorps AU040643, anticorps AW060375, anticorps ILC, anticorps CTACK, anticorps ESkine, anticorps Akp2, anticorps alp, anticorps zgc:56672, anticorps DDBDRAFT_0205491, anticorps DDBDRAFT_0231570, anticorps DDB_0205491, anticorps DDB_0231570, anticorps Actn2lp, anticorps Alp, anticorps asparaginase like 1, anticorps bacterial alkaline phosphatase, anticorps C-C motif chemokine ligand 27, anticorps alkaline phosphatase, liver/bone/kidney, anticorps alkaline phosphatase, anticorps PDZ and LIM domain 3, anticorps ASRGL1, anticorps phoA, anticorps Asrgl1, anticorps CCL27, anticorps alpl, anticorps alp, anticorps Pdlim3

Sujet:

Synonyms: Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody

Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).

Gene Name: phoA

ID gène: 945041

UniProt: P00634