Ferritin anticorps (HRP)

N° du produit ABIN5596710

Détail du produit anti-Ferritin anticorps (HRP)

Antigène: Ferritin (FE)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ferritin est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Réactivité croisée (Details): Cross reactivity against Ferritin from other sources is unknown.

Purification: Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Immunogène:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Ferritin (Human Spleen) Antibody has been assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Commentaires:

User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Ferritin

Antigène: Ferritin (FE)

Autre désignation: Ferritin (FE Produits)

Synonymes: anticorps 1HCH, anticorps BEST:LD36673, anticorps CG2216, anticorps Dmel\\CG2216, anticorps FER1, anticorps FER1HCH, anticorps Fer-L, anticorps Fer1, anticorps Fer1HC, anticorps FerHCH, anticorps HCH, anticorps T21, anticorps T9, anticorps fer1, anticorps fer1hch, anticorps l(3)00451, anticorps l(3)j10B4, anticorps SFerH-1, anticorps SOF-35, anticorps SOF-5L, anticorps NV13011, anticorps ACP1, anticorps F8A5.24, anticorps F8A5_24, anticorps Ferritin 1 heavy chain homologue, anticorps ferritin light chain, anticorps ferritin, anticorps chromosome 26 open reading frame, human C10orf131, anticorps hyaluronan and proteoglycan link protein 2, anticorps NAD(P)-linked oxidoreductase superfamily protein, anticorps Fer1HCH, anticorps H-1, anticorps YPDSF_1340, anticorps ftnA, anticorps Ft, anticorps C26H10orf131, anticorps HAPLN2, anticorps AT1G60730

Sujet:

Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.

ID gène: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis