Fibronectin anticorps (Biotin)

N° du produit ABIN5596762

Détail du produit anti-Fibronectin anticorps (Biotin)

Antigène: Fibronectin

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Réactivité croisée (Details): Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

Purification: Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity.

Immunogène:

Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Informations sur le produit:

Cet anticorps polyclonal de lapin anti-fibronectine est conjugué à la biotine et convient à la détection de la fibronectine (FN, FN1) chez l'homme, la souris, le rat, le bovin et le singe. L'anticorps anti-fibronectine est validé pour l'ELISA, l'immunohistochimie, l'immunoprécipitation et le western blotting. Quatre images du produit pour l'IHC et le profil de dépliage sont actuellement disponibles comme preuve de la performance de l'anticorps. Utilisez notre anticorps de haute qualité contre la Fibronectine pour détecter de manière fiable la Fibronectine pour une variété d'espèces.

Les fibronectines se lient aux surfaces cellulaires et à divers composés dont le collagène, la fibrine, l'héparine, l'ADN et l'actine. Les fibronectines sont impliquées dans l'adhésion cellulaire, la motilité cellulaire, l'opsonisation, la cicatrisation et le maintien de la forme des cellules. Impliquée dans la compaction des ostéoblastes par le processus d'assemblage matriciel médié par les cellules de fibronectine, essentiel pour la minéralisation des ostéoblastes (Par similarité). Participe à la régulation du dépôt de collagène de type I par les ostéoblastes (par similarité). (UniProt)

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-fibronectin (rabbit) antibody was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Independent Validation (UP)

Validation #104084 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104084

Date: 23.07.2019

Antigène: Fibronectin

Numéro du lot: 39102

Application validée: Unfolding Profile

Contrôle positif: ABIN5596762

Conclusion:

Passed. ABIN5596762 showed T_i at 63.4°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN5596762. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 63.4°C.

Protocole

Full Protocol:

• Dilute ABIN5596762 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.5µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Fibronectin

Antigène: Fibronectin

Abstract: Fibronectin Produits

Synonymes: anticorps FN, anticorps cig, anticorps fibronectin, anticorps finc, anticorps lets, anticorps msf, anticorps CIG, anticorps ED-B, anticorps FINC, anticorps FNZ, anticorps GFND, anticorps GFND2, anticorps LETS, anticorps MSF, anticorps E330027I09, anticorps Fn, anticorps Fn-1, anticorps FIBNEC, anticorps fn-1, anticorps fibronectin 1, anticorps FN1, anticorps fn1, anticorps Fn1

Sujet:

Synonyms: CIG antibody, Cold insoluble globulin antibody, LETS antibody, Migration stimulating factor antibody, MSF antibody, Transformation sensitive protein. Antibody

Background: Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin, an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.

Gene Name: FN1

ID gène: 2335

UniProt: P02751