Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Cholesterol Oxidase anticorps (HRP)

choS Reactivité: Streptomyces ELISA, WB, IP Hôte: Chèvre Polyclonal HRP
N° du produit ABIN5596764
  • Antigène Tous les produits Cholesterol Oxidase (choS)
    Cholesterol Oxidase (choS)
    Reactivité
    • 5
    • 4
    • 4
    • 1
    Streptomyces
    Hôte
    • 12
    • 2
    Chèvre
    Clonalité
    • 14
    Polyclonal
    Conjugué
    • 5
    • 5
    • 2
    • 2
    Cet anticorp Cholesterol Oxidase est conjugé à/à la HRP
    Application
    • 14
    • 10
    • 4
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Cross reactivity against Cholesterol Oxidase from other sources is unknown.
    Purification
    Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].
    Immunogène

    Immunogen: Cholesterol Oxidase [Streptomyces]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Indications d'application

    Application Note: Anti-Cholesterol Oxidase has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Gentamicin sulfate
    Précaution d'utilisation
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Cholesterol Oxidase (choS)
    Autre désignation
    Cholesterol Oxidase (choS Produits)
    Synonymes
    anticorps SCD63.13, anticorps cholesterol oxidase, anticorps GMC family oxidoreductase, anticorps Cholesterol oxidase, anticorps choS, anticorps SCO4781, anticorps BTH_I0619, anticorps SARE_RS13770, anticorps Lcho_3191, anticorps Npun_R6526, anticorps Amir_2425, anticorps Caci_5961, anticorps Sros_4246, anticorps Kfla_6484, anticorps Snas_3535, anticorps Weevi_2091
    Sujet

    Synonyms: choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

    Background: Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

    Gene Name: choA

    ID gène
    1215660
    UniProt
    P12676
Vous êtes ici:
Support technique