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Pyruvate Kinase anticorps (HRP)

PK Reactivité: Lapin WB, ELISA Hôte: Chèvre Polyclonal HRP
N° du produit ABIN5596779
  • Antigène Voir toutes Pyruvate Kinase (PK) Anticorps
    Pyruvate Kinase (PK)
    Reactivité
    • 9
    • 8
    • 7
    • 4
    • 3
    Lapin
    Hôte
    • 8
    • 5
    • 4
    Chèvre
    Clonalité
    • 17
    Polyclonal
    Conjugué
    • 14
    • 3
    Cet anticorp Pyruvate Kinase est conjugé à/à la HRP
    Application
    • 15
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Réactivité croisée (Details)
    This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.
    Purification
    Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].
    Immunogène

    Immunogen: Pyruvate Kinase [Rabbit Muscle]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product PK Anticorps primaire
  • Indications d'application

    Application Note: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:4,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Gentamicin sulfate
    Précaution d'utilisation
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Pyruvate Kinase (PK)
    Autre désignation
    Pyruvate Kinase (PK Produits)
    Synonymes
    anticorps CG7070, anticorps Dmel\\CG7070, anticorps PYK, anticorps Pyk, anticorps PYK3b, anticorps PYK3c, anticorps Pyruvate kinase, anticorps PK, anticorps pyruvate kinase, anticorps pyruvate kinase (PK), anticorps Pyruvate kinase (PK), anticorps pyruvate kinase PKM, anticorps testis expressed 45, anticorps PyK, anticorps pykA, anticorps PYK3a|PYK3b|PYK3c, anticorps pyk, anticorps pyk1, anticorps pk, anticorps LOC100534543, anticorps TEX45
    Sujet

    Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, HRP

    Background: Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.

    Gene Name: PKM2

    ID gène
    100008676
    UniProt
    P11974
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