Streptavidin anticorps (Biotin)

N° du produit ABIN5596791

Détail du produit anti-Streptavidin anticorps (Biotin)

Antigène: Streptavidin

Reactivité: Streptomyces avidinii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Streptavidin est conjugé à/à la Biotin

Application: ELISA

Réactivité croisée (Details): No reaction was observed against Avidin.

Purification: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.

Immunogène:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentation in immunoassays should be performed by the researcher.

ELISA Dilution: 1:2,000-1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Streptavidin

Antigène: Streptavidin

Abstract: Streptavidin Produits

Sujet:

Synonyms: BAC, Streptavidin Biotin, Anti-Streptavidin BAC Antibody

Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin thorught the antbody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interation.