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Streptavidin anticorps (Biotin)

Reactivité: Streptomyces avidinii ELISA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN5596791
  • Antigène Voir toutes Streptavidin Anticorps
    Streptavidin
    Reactivité
    • 32
    • 16
    • 4
    • 2
    • 2
    Streptomyces avidinii
    Hôte
    • 38
    • 13
    • 3
    Lapin
    Clonalité
    • 41
    • 12
    Polyclonal
    Conjugué
    • 21
    • 8
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Streptavidin est conjugé à/à la Biotin
    Application
    • 28
    • 21
    • 13
    • 13
    • 13
    • 10
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA
    Réactivité croisée (Details)
    No reaction was observed against Avidin.
    Purification
    Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.
    Immunogène

    Immunogen: Streptavidin (Streptomyces avidinii)

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product Streptavidin Anticorps primaire
  • Indications d'application

    Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentation in immunoassays should be performed by the researcher.

    ELISA Dilution: 1:2,000-1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Streptavidin
    Abstract
    Streptavidin Produits
    Sujet

    Synonyms: BAC, Streptavidin Biotin, Anti-Streptavidin BAC Antibody

    Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin thorught the antbody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interation.

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