Acid Phosphatase anticorps (HRP)
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- Antigène Voir toutes Acid Phosphatase (ACP) Anticorps
- Acid Phosphatase (ACP)
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Reactivité
- Potato
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Acid Phosphatase est conjugé à/à la HRP
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Application
- Western Blotting (WB), ELISA
- Réactivité croisée (Details)
- Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
- Purification
- Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
- Immunogène
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Immunogen: Acid Phosphatase [Potato]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Indications d'application
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Application Note: Anti-Acid Phosphatase is suitable for use in ELISA, western blot, and immunohistochemistry. Optimal titers should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Agent conservateur
- Gentamicin sulfate
- Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- Acid Phosphatase (ACP)
- Autre désignation
- Acid Phosphatase (ACP Produits)
- Synonymes
- anticorps Acph, anticorps ACP, anticorps Acid phosphatase, anticorps acid phosphatase, anticorps cerebral palsy, ataxic 1, anticorps Dana\Acph, anticorps phoC, anticorps CPAT1
- Sujet
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Synonyms: Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
Background: Acid Phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function, therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used to evaluate the success of the surgical treatment of prostate cancer.
Gene Name: PAP3
- UniProt
- Q6J5M8
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