COL2 anticorps

N° du produit ABIN5596824

Détail du produit anti-COL2 anticorps

Antigène: COL2 (Collagen, Type II (COL2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mammifères, Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée (Details): Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogène:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotype: IgG

Informations sur le produit:

À quoi peut servir l'anticorps COL2 ABIN5596824 ? Cet anticorps polyclonal non conjugué de lapin COL2 convient à la détection du collagène de type 2 (COL2) par ELISA, immunohistochimie, immuniprécipitation et Western blotting. L'anticorps reconnaît COL2 chez l'homme, la souris, le rat, le bovin, le mouton et d'autres mammifères.

Quelles sont les données de validation disponibles pour cet anticorps COL2 ? Le produit a été validé indépendamment par NanoTemper Technologies en déterminant le profil de dépliage. Il y a actuellement 2 images de produit disponibles montrant les performances de l'anticorps COL2. Utilisez notre anticorps pour détecter COL2 de manière fiable.

Quelle est la fonction de COL2 ? Le collagène de type 2 (COL2) est spécifique des tissus cartilagineux. Il est essentiel pour le développement embryonnaire normal du squelette, pour la croissance linéaire et pour la capacité du cartilage à résister aux forces de compression. L'anticorps COL2 a pour fonction de détecter le collagène de type 2.

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

Restrictions: For Research Use only

Independent Validation (UP)

Validation #104083 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104083

Date: 23.07.2019

Antigène: COL2

Numéro du lot: 41639

Application validée: Unfolding Profile

Contrôle positif: ABIN5596824

Conclusion:

Passed. ABIN5596824 showed T_i at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.

Protocole

Full Protocol:

• Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur COL2

Antigène: COL2 (Collagen, Type II (COL2))

Autre désignation: Collagen Type II (COL2 Produits)

Synonymes: anticorps PT, anticorps RPRGL2, anticorps THPH1, anticorps Cf-2, anticorps Cf2, anticorps FII, anticorps thrombin, anticorps prothrombin, anticorps CAP18, anticorps Cnlp, anticorps Cramp, anticorps FALL39, anticorps MCLP, anticorps coagulation factor II, thrombin, anticorps coagulation factor II, anticorps cathelicidin antimicrobial peptide, anticorps Cuticle collagen 2, anticorps collagen type II alpha 1 chain, anticorps F2, anticorps Camp, anticorps col-2, anticorps COL2A1

Sujet:

Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. We extensively purify collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL2A1

ID gène: 1280

UniProt: P02458