Collagen Type V anticorps (Biotin)
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- Antigène Voir toutes Collagen Type V (COL5) Anticorps
- Collagen Type V (COL5) (Collagen, Type V (COL5))
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Reactivité
- Humain, Boeuf (Vache), Mammifères
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Collagen Type V est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Réactivité croisée (Details)
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Purification
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- Immunogène
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Immunogen: Collagen Type V from human and bovine placenta
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product COL5 Anticorps primaire
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- Indications d'application
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Immunohistochemistry Dilution: 1:50 to 1:200
Application Note: This product was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Collagen V in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. This product was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Collagen V in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200.
Western Blot Dilution: 1:5,000 to 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 to 1:8,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.1 mg/mL
- Buffer
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- Date de péremption
- 12 months
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Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." dans: Blood, Vol. 122, Issue 7, pp. 1105-13, (2013) (PubMed).
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Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." dans: Blood, Vol. 122, Issue 7, pp. 1105-13, (2013) (PubMed).
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- Antigène
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- Autre désignation
- Collagen Type V (COL5 Produits)
- Sujet
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Synonyms: COL5A2 protein antibody, Col5A3 antibody, Collagen fetal membrane A polypeptide antibody
Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
Gene Name: COL5A1
- ID gène
- 50509
- UniProt
- P25940
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