Esterase anticorps (HRP)

N° du produit ABIN5596851

Détail du produit anti-Esterase anticorps (HRP)

Antigène: Esterase

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Esterase est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.

Purification: Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].

Immunogène:

Immunogen: Esterase [Porcine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Esterase has been assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Esterase

Antigène: Esterase

Abstract: Esterase Produits

Synonymes: anticorps Carboxylic ester hydrolase, anticorps T28C12.4, anticorps K11G9.1, anticorps K07C11.4, anticorps F15A8.6, anticorps K11G9.2, anticorps K11G9.3, anticorps T28C12.5

Sujet:

Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.

Gene Name: CES3

ID gène: 100626873

UniProt: Q29550