Myosin anticorps (N-Term, pSer19, pSer20)

N° du produit ABIN5596872

Détail du produit anti-Myosin anticorps

Antigène: Myosin

Épitope: N-Term, pSer19, pSer20

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myosin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée (Details): Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

Immunogène:

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Myosin

Antigène: Myosin

Abstract: Myosin Produits

Synonymes: anticorps CG3201, anticorps DmMLC_C, anticorps Dmel\\CG3201, anticorps MyoII, anticorps mlc-c, anticorps nmELC, anticorps MYOSIN, anticorps myosin 1, anticorps DFNA4, anticorps DFNA4A, anticorps MHC16, anticorps MYH17, anticorps NMHC II-C, anticorps NMHC-II-C, anticorps PNMHH, anticorps myosin, anticorps MYH1, anticorps MYH2, anticorps MYH3, anticorps MYH4, anticorps MYH8, anticorps adult, anticorps embryonic, anticorps 2400004E04Rik, anticorps II-C, anticorps NHMCII, anticorps DROMHC, anticorps Dm nmII, anticorps DmnmII, anticorps Dronm-MII, anticorps E(br), anticorps MHC, anticorps Mhc-c, anticorps Mhc-c[1], anticorps Myo, anticorps Myo II, anticorps NMM, anticorps Zip, anticorps anon-WO0140519.37, anticorps l(2)02957, anticorps nmMHC, anticorps nmy-2, anticorps zpr, anticorps DmelCG15792, anticorps CG15792, anticorps Myosin light chain cytoplasmic, anticorps hypothetical protein, anticorps myosin 1, anticorps myosin heavy chain 9, anticorps myosin heavy chain 14, anticorps myosin, heavy chain 1B, skeletal muscle, anticorps myosin, heavy polypeptide 14, anticorps zipper, anticorps Mlc-c, anticorps T22C1.6, anticorps ATM1, anticorps MYH9, anticorps MYH14, anticorps Myh14, anticorps MYH1B, anticorps zip

Sujet:

Synonyms: Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2, nonsarcomeric, Myosin RLC, MLC-2B,

Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Gene Name: MYL12A

ID gène: 10627

UniProt: P19105