Phosphothreonine anticorps (pThr) (Biotin)

N° du produit ABIN5596877

Détail du produit anti-Phosphothreonine anticorps (Biotin)

Antigène: Phosphothreonine

Épitope: pThr

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Phosphothreonine est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.

Purification: Anti-PhosphoThreonine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Immunogène:

Immunogen: PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: PhosphoThreonine affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store anti-pT antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Phosphothreonine

Antigène: Phosphothreonine

Abstract: Phosphothreonine Produits

Classe de substances: Amino Acid

Sujet:

Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Background: Anti-PhosphoThreonine antibodies are broadly reactive allowing these antibodies especially valuable in multiplex analyses and target discovery programs