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Penicillinase anticorps (HRP)

Reactivité: Enterobacter cloacae WB, IP, ELISA Hôte: Lapin Polyclonal HRP
N° du produit ABIN5596958
  • Antigène Tous les produits Penicillinase
    Penicillinase
    Reactivité
    • 4
    • 1
    Enterobacter cloacae
    Hôte
    • 4
    • 1
    Lapin
    Clonalité
    • 4
    • 1
    Polyclonal
    Conjugué
    • 4
    • 1
    Cet anticorp Penicillinase est conjugé à/à la HRP
    Application
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Réactivité croisée (Details)
    Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
    Purification
    Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    Immunogène

    Immunogen: Penicillinase [Enterobacter cloacae]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Indications d'application

    Application Note: Anti-Penicillinase has been assayed against 1.0 μg of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Gentamicin sulfate
    Précaution d'utilisation
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Penicillinase
    Abstract
    Penicillinase Produits
    Synonymes
    anticorps beta-lactamase TEM-1, anticorps blaTEM-1
    Sujet

    Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody

    Background: Penicillinase is a specific type of β-lactamase, showing specificity for penicillins, again by hydrolysing the beta-lactam ring. Molecular weights of the various penicillinases cluster around 50 kDa. Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a beta-lactam. Through hydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.

    Gene Name: OXA9

    ID gène
    9125732
    UniProt
    P05364
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