R-Phycoerythrin anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN5596963

Détail du produit anti-R-Phycoerythrin anticorps (Alkaline Phosphatase (AP))

Antigène: R-Phycoerythrin (Phycoerythrin, R-)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp R-Phycoerythrin est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Références pour anticorps anti-R-Phycoerythrin (Alkaline Phosphatase (AP)) (ABIN5596963)

Beute, Lukkes, Koekoek, Nastiti, Ganesh, de Bruijn, Hockman, van Nimwegen, Braunstahl, Boon, Lambrecht, Manganiello, Hendriks, KleinJan: "A pathophysiological role of PDE3 in allergic airway inflammation." dans: JCI insight, Vol. 3, Issue 2, (2018) (PubMed).

Détails sur R-Phycoerythrin

Antigène: R-Phycoerythrin (Phycoerythrin, R-)

Autre désignation: R-Phycoerythrin (R-Phycoerythrin Produits)

Classe de substances: Chemical

Sujet:

Synonyms: ALP, Alk Phos