ENO2/NSE anticorps (AA 416-433)
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- Antigène Voir toutes ENO2/NSE (ENO2) Anticorps
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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Épitope
- AA 416-433
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ENO2/NSE est non-conjugé
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Application
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Attributs du produit
- This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
- Purification
- Purified
- Pureté
- Protein G affinity chromatography
- Immunogène
- Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
- Clone
- ENO2-1462
- Isotype
- IgG2b
- Top Product
- Discover our top product ENO2 Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Neuron Specific Enolase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Autre désignation
- NSE / Neuron Specific Enolase (ENO2 Produits)
- Synonymes
- anticorps ENO2, anticorps DKFZp459B1817, anticorps NSE, anticorps AI837106, anticorps D6Ertd375e, anticorps Eno-2, anticorps RNEN3, anticorps eno3, anticorps wu:fc09h05, anticorps zgc:92418, anticorps enolase 2, anticorps enolase 2 (gamma, neuronal), anticorps enolase 2, gamma neuronal, anticorps enolase 2, gamma, neuronal, anticorps ENO2, anticorps Eno2, anticorps eno2
- Sujet
- This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
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